Protein Separation

Protein Separation

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With the current wave of interest in biomacromolecular products, adsorption technologies to separate and purify biomolecules from their production environment are also evolving. Common adsorption methods, including ion-exchange chromatography, affinity chromatography, hydrophobic chromatography, and reversed-phase chromatography, cannot handle contaminants such as cell debris. Alfa Chemistry can use colloidal magnetic nano-ion adsorption separation technology to achieve effective separation of proteins.

Protein Separation

Our Separation Solutions

Alfa Chemistry enables the preparation of specialized nanoparticles coated with self-assembled surfactants and lipid layers. These nanoparticles offer large interfacial areas, small diffusion resistance, and high selectivity and capacity for large biological products such as proteins.

Protein SeparationFig.1 Scheme of selective protein adsorption onto nanoparticles (NPs) leading to immune escape and specific targeting.[1]

The protein adsorption scheme of magnetic nanoparticles is as follows:

1. Mix the protein solution with the magnetic nanoparticles and incubate for a certain time at room temperature.

2. Use high gradient magnetic filtration to separate proteins that are not adsorbed on the particles from the medium.

3. Quantify free protein using a UV-Vis spectrophotometer and gel electrophoresis, and determine the amount of protein adsorbed by the difference between the known feed amount and the total mass of free protein in the recovered filtrate.

4. The desorption of the adsorbed protein is achieved by adjusting the pH value.


What We Offer?

  • Synthesis of Phospholipid-Coated Magnetic Nanoparticles

We were able to synthesize surfactant-coated nanoparticles using chemical co-precipitation. Then, the magnetic liposomes are obtained by performing dialysis, ultrasound, magnetic filtration and other steps on the mixture of magnetic nanoparticles and phospholipid vesicles.

Protein SeparationFig.2 Schematic illustration of a coated magnetic nanoparticle showing the relative sizes of the particle core, lipid coating and adsorbed protein.[2]

  • Colloidal stability testing of nanoparticle suspensions

We can evaluate the temperature stability of colloidal suspensions by adjusting the pH and heating.

  • Protein Adsorption & separation of magnetic nanoparticles

We are able to provide separation of binary and ternary protein mixtures and use high gradient magnetic separators to separate magnetic particles of unadsorbed proteins from the mixture.


Our Service Advantages

  • Alfa Chemistry's innovative adsorption technology enables highly selective and high-capacity separation of proteins.
  • Alfa Chemistry's technical team has rich resources and many advanced technologies, and has strong independent development capabilities.
  • Alfa Chemistry has always expanded our business areas closely around the core strategy of "providing excellent services".
  • Alfa Chemistry maintains good cooperative relations with many well-known universities and enterprises.

References

  1. Roberta Cagliani, et al. Protein Adsorption: A Feasible Method for Nanoparticle Functionalization? Materials. 2019.
  2. Bucak S, et al. Protein separations using colloidal magnetic nanoparticles. Biotechnology Progress. 2010, 19(2):477-484.

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