Protein A - 50nm Silver Conjugate

Protein A - 50nm Silver Conjugate

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Typical Properties

CatalogACCM0134
Gold Nanoparticle Core Size50 nm
Absorption Max415-430 nm
Optical Density7.5 OD
Conjugated ProteinProtein A (extracellular), from S. aureus
Working Dilution1:5-1:100
Supplied in1XPBS (pH 7.4), 20% glycerol (v/v), 1% bovine serum albumin (BSA)

Quantity You Can Choose

Quantity0.5 ml, 1 ml

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Overview of Protein A - 50nm Silver Conjugate

Description

Applications

Storage

Description

Protein A - 50nm silver conjugate represents a powerful tool for immunological research and diagnostics. The conjugate consists of Protein A molecules covalently attached to 50 nm silver nanoparticles. Protein A is a bacterial cell wall protein that has a high affinity for the Fc region of immunoglobulins, particularly immunoglobulin G (IgG). Silver nanoparticles exhibit strong plasmonic properties, providing a highly sensitive signal amplification platform. Protein A - 50nm silver conjugate combines the specific binding ability of Protein A with the signal amplification properties of silver nanoparticles, allowing Fc-containing antibodies to be easily and quickly bound to the silver surface, which can then be further utilized downstream.

Applications

Protein A - 50nm silver conjugate allows for sensitive and specific detection of antibodies and antigens in a variety of applications. For example, it can be used in immunohistochemistry (IHC) to visualize specific proteins in tissue samples. It can also be used in enzyme-linked immunosorbent assays (ELISA) and Western blotting to detect and quantify specific proteins. In addition, it can also be used in flow cytometry to detect cell surface antigens by binding to specific antibodies. Moreover, Protein A - 50nm silver conjugate can also be used in affinity chromatography to purify antibodies or other proteins by binding to the Fc region of immunoglobulins.

Storage

Store undiluted in storage buffer at 2-8 °C. Stable for 4 months if stored as specified.

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