Protein A - 50nm Gold Conjugate

Protein A - 50nm Gold Conjugate

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Typical Properties

CatalogACCM0055
Gold Nanoparticle Core Size50 nm
Absorption Max535 nm
Conjugated ProteinProtein A (extracellular), from S. aureus
Working Dilution1:5-1:100
Supplied in1XPBS (pH 7.4), 20% glycerol (v/v), 1% bovine serum albumin (BSA)

Size, Optical Density and Status You Can Choose

Size0.5 ml, 1 ml
Optical Density3 OD, 10 OD
StatusLyophilized or Not Lyophilized

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Overview of Protein A - 50nm Gold Conjugate

Description

Applications

Storage

Description

Protein A - 50nm gold conjugate is a valuable tool for researchers in the fields of immunology and cell biology. The conjugate consist of Protein A, a bacterial cell wall protein that has high affinity for the Fc portion of immunoglobulins (specifically IgG), linked to colloidal gold particles. Protein A - 50nm gold conjugate allows for the convenient and rapid conjugation of Fc-containing antibodies to gold surfaces, providing a versatile and reliable method for the detection and visualization of specific proteins, antigens or molecules in biological samples. Compared with traditional immunohistochemistry techniques, it has higher sensitivity and specificity and can recognize multiple antigens simultaneously.

Applications

  • Immunohistochemistry. Protein A - 50nm gold conjugate is commonly used in immunohistochemistry studies to detect and localize specific proteins, antigens or molecules in tissues and cells. The gold nanoparticles provide a visible signal that can be easily detected under a microscope.
  • Protein purification. Protein A - 50nm gold conjugate can be used for the purification of antibodies or other proteins. Protein A can bind to the Fc region of antibodies with high specificity, thus facilitating the isolation and purification of specific proteins.
  • Diagnostic assays. Protein A - 50nm gold conjugate can also be used in diagnostic assays for specific antibodies or antigens.

Storage

  • Store undiluted in storage buffer at 2-8 °C. Stable for 4 months if stored as specified.
  • It is important to note that storing conjugate at working dilution may result in performance loss.

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