Gelatin solution

Typical Properties
CAS	9000-70-8
Catalog	ACM9000708-13
Melting Point	> 226 °C (dec.)
Purity	≥70 % protein basis (biuret)
Density	1.2 g/cm3
Solubility	H2O: 67 mg/mL at 50 °C, slightly hazy, slightly yellow
Appearance	Liquid
Storage	2-8 °C
Biological Source	Bovine Hide
Cation Traces	Ca: ≤2000 mg/kg Cd: ≤5 mg/kg Co: ≤5 mg/kg Cr: ≤10 mg/kg Cu: ≤50 mg/kg Fe: ≤50 mg/kg K: ≤500 mg/kg Mg: ≤500 mg/kg Mn: ≤5 mg/kg Na: ≤5000 mg/kg Ni: ≤5 mg/kg Pb: ≤5 mg/kg Zn: ≤10 mg/kg
Color	White to pale yellow
Composition	(C6H12O6)n
Concentration	2 % in H2O, tissue culture grade
EC Number	232-554-6
Grade	Reagent Grade
Loss	< 14 % loss on drying
MDL Number	MFCD00081638
Mesh	8-60 Mesh
pH	4.0-7.0 at 66.7 g/L at 60 °C
Precautions for use	Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.
Quality Level	200
Shipping	Ambient
Stability	Sensitive to light
Transmittance	90 %

Overview

Description

Gelatin is a hydrocolloid and is rich in glycine, proline and hydroxyproline, which imparts structural stability. It is synthesized from the alkaline digestion of collagen from bovine skin and is referred as type B. Gelatin has wide applications in food industry. It takes up random coil structure after digestion from the triple helical collagen. The most common source for industrial production of gelatin is slaughter byproducts. The type A gelatin and type B differ in their isoelectric pH. This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objective.

Features

·Good biocompatibility
·Heat reversible freezing
·Wide range of applications
·High security
·Green and environmentally friendly
·Batch stable

Application

·As a blocking reagent in Western blotting, ELISA and immunochemistry,
·As a component of medium for species differentiation in bacteriology
·As a delivery vehicle for the release of active biomolecules and the generation of scaffolds for tissue engineering applications
·Use as a suspending and encapsulating agent, among other applications