Colloidal Gold Immunohistochemistry

Introduction

As a gold hydrosol, colloidal gold can rapidly adsorb proteins without affecting the biological activity of proteins, and is an ideal labeling material. The mechanism of colloidal gold labeling is based on the electrostatic adsorption between the negative charges on the surface of colloidal gold particles and the positively charged groups of proteins. Compared with radioisotope, fluorescein and enzyme labeling techniques, immunocolloidal gold technology has the following advantages: (a) the preparation process of colloidal gold is simple and the size is controllable; (b) the non-specific adsorption of colloidal gold on tissue cells is small; (c) Double and multiple labeling can be carried out, and double or multiple labeling can be carried out in combination with other markers; (d) A variety of biological macromolecules can be labeled; (e) It can be used for both light microscopy and electron microscopy; (f) High sensitivity, easy staining and long-term preservation of results; (g) The experimental results can be qualitative and quantitative.^[1]

Fig.1 Electron microscopy of reticulocyte internal vesicles labelled with colloidal gold-transferrin.^[2]

Our Services

Our services include the following:

• Preparation and purification of colloidal gold
  We can prepare colloidal gold particles with different particle sizes by chemical reduction method (commonly used reducing agents include chloroauric acid, white phosphorus, ascorbic acid, trisodium citrate, tannic acid-trisodium citrate, etc.). Colloidal gold can also be prepared into different immunogold probes and purified by ultracentrifugation, gel filtration or gradient centrifugation to remove unbound proteins, unstable colloidal gold and polymers formed during the labeling process.
• Immunolight microscopy
  We can use the immunogold and silver method (IGSS) to detect antigens in living tissue or tissue sections at the light microscope level, and to detect antigens on the surface of cultured cells and in cells.
• Immunoelectron microscopy
  Our immunoelectron microscopy technology can meet the detection of multiple markers and genes at the same time, including chromosomal gene localization, determination of the distribution of specific nucleic acid sequences in cells, detection of gene expression, analysis of nucleic acid replication process, etc.

Fig.2 High-power electron micrograph of rat pituitary immunolabeled using an immunogold technique.^[4]

Typical Applications of Colloidal Gold Immunohistochemistry

References

1. Sarengaowa, et al. Colloidal Gold Immunoelectron Microscopy Technique And Its Application. Journal of Inner Mongolia Medical University. 2007.
2. C Géminar d, et al. Reticulocyte maturation: mitoptosis and exosome release. Biocell. 2002.
3. Junyan Chen and Qiong Xiao. Application and Prospect of Immune Colloidal Gold Technique. Chemical Analysis and Meterage. 2014.
4. Sarraf C E. Immunolabeling for Electron Microscopy. Methods in Molecular Medicine. 2000, 40:439-452.

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