The continuous development of colloidal gold labeling technology has greatly expanded its application range, especially in the field of immunohistochemistry. Alfa Chemistry can provide colloidal gold immunohistochemistry services at the level of light microscopy and electron microscopy. Our service supports dual or multi-labeling, enabling simultaneous observation of the localization of different antigens and receptors on cell surfaces and cellular structures. We provide customers with a complete sample preparation and testing process.
As a gold hydrosol, colloidal gold can rapidly adsorb proteins without affecting the biological activity of proteins, and is an ideal labeling material. The mechanism of colloidal gold labeling is based on the electrostatic adsorption between the negative charges on the surface of colloidal gold particles and the positively charged groups of proteins. Compared with radioisotope, fluorescein and enzyme labeling techniques, immunocolloidal gold technology has the following advantages: (a) the preparation process of colloidal gold is simple and the size is controllable; (b) the non-specific adsorption of colloidal gold on tissue cells is small; (c) Double and multiple labeling can be carried out, and double or multiple labeling can be carried out in combination with other markers; (d) A variety of biological macromolecules can be labeled; (e) It can be used for both light microscopy and electron microscopy; (f) High sensitivity, easy staining and long-term preservation of results; (g) The experimental results can be qualitative and quantitative.
Fig.1 Electron microscopy of reticulocyte internal vesicles labelled with colloidal gold-transferrin.
Our services include the following:
Fig.2 High-power electron micrograph of rat pituitary immunolabeled using an immunogold technique.
If you are interested in our Colloidal Gold Immunohistochemistry, please don't hesitate to place an order now!