Anti-6X His - 10nm Gold Conjugate

Anti-6X His - 10nm Gold Conjugate

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Typical Properties

CatalogACCM0038
Gold Nanoparticle Core Size10 nm
Absorption Max515-520 nm
Conjugated ProteinMouse anti-6X His antibody
Working Dilution1:1-1:100
Supplied in1XPBS (pH 7.4), 20% glycerol (v/v), 1% bovine serum albumin (BSA)

Size and Optical Density You Can Choose

Size0.5 ml, 1 ml
Optical Density3 OD, 10 OD

*If you have other needs, please feel free to contact us.

Overview of Anti-6X His - 10nm Gold Conjugate

Description

Applications

Storage

Description

The anti-6X His - 10nm gold conjugate consists of gold nanoparticles that are functionalized with an anti-6X His antibody. 6X His is a histidine tag consisting of a series of histidine residues at the amino or carboxyl terminus of a protein. Anti-6X His - 10nm gold conjugate can specifically recognize the amino acid sequence of the 6X His tag, thereby detecting and quantifying proteins with the 6X His tag.

Applications

  • One of the main applications of anti-6X His - 10nm gold conjugate is in the detection and visualization of proteins in cells and tissues. It can be used in techniques such as immunofluorescence, electron microscopy, Western blot, and enzyme-linked immunosorbent assay (ELISA) to accurately localize and quantify protein expression. The use of gold nanoparticles in this conjugate offers several advantages, including their small size, high stability, and unique optical properties, making them ideal for various imaging and detection techniques.
  • Additionally, anti-6X His - 10nm gold conjugate can also be utilized in protein purification assays, where it can efficiently capture and isolate tagged proteins from complex mixtures, thereby improving the purity of target proteins.

Storage

  • Store undiluted in storage buffer at 2-8 °C. Stable for 4 months if stored as specified.
  • It is important to note that storing conjugate at working dilution may result in performance loss.

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