Animal & Plant Quarantine

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Viruses can pose a significant threat to animal, plant and human health, so rapid and sensitive virus detection is critical before effective treatment. Currently, detection methods based on gold nanoparticles are considered to have great potential. Alfa Chemistry can provide animal and plant quarantine services based on immunocolloidal gold technology, including the detection of important animal viral diseases, bacterial diseases and common zoonotic diseases.

Animal and Plant Quarantine

Introduction

Rapid and accurate virus detection is of great significance for assessing the infection status and controlling the spread of the virus. Therefore, for such applications, highly sensitive and reliable diagnostic tools are required. Traditional virus isolation and serological testing are laborious, time-consuming, and have low sensitivity. PCR-based diagnostic techniques, on the other hand, require expensive instruments and relatively complex laboratories. In contrast, the colloidal gold test strip method, which provides fast and reliable results with little equipment dependence, is a promising method.

Fig.1 Schematic diagram of colloidal gold for virus detection.^[1]

Our Detection Range

Alfa chemistry is able to integrate expertise related to virus detection and develop immunocolloidal gold-based assays to more effectively combat various infectious diseases.

Our virus detection range includes but is not limited to:

Animal virus	Avian leukemia virus, Lymphocyst virus, Porcine reproductive and respiratory syndrome virus, Influenza virus, Severe fever with thrombocytopenia syndrome virus, Shrimp white spot syndrome virus, Foot-and-mouth disease virus, Duck enteritis virus, African swine fever virus, Human norovirus , Rotavirus, Coronavirus, Hepatitis virus, Herpes virus, Newcastle disease virus, Parvovirus, Dengue virus, Rabies virus, etc.
Plant virus	Rice streak virus, Plum pox virus, Barley yellow dwarf virus, Ring spot virus, etc.
Bacteria	Salmonella, Syphilis, Brucella species, Vibrio cholerae, Bacillus anthracis, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus aureus, Listeria, Campylobacter jejuni, etc.
Parasite	Cryptosporidium, Theileria sheep, Plasmodium, Clonorchis sinensis, Toxoplasma gondii, etc.

Our Service Process

Antibody production and purification
Synthesis and identification of colloidal gold
Preparation of colloidal gold-antibody probes
Preparation of immunochromatographic test strips
Virus detection
Sensitivity and specificity testing of immunochromatographic strips
Reproducibility and stability testing of immunochromatographic strips

References

Wang J, et al. Gold nanoparticles in virus detection: Recent advances and potential considerations for SARS-CoV-2 testing development. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology. 2021.
A J Y, et al. Development and application of a colloidal gold test strip for the rapid detection of the infectious laryngotracheitis virus - ScienceDirect. Poultry Science. 2020, 99( 5):2407-2415.
X. Z. Sheng, et al. Development of a colloidal gold immunochromatographic test strip for detection of lymphocystis disease virus in fish. Journal of Applied Microbiology. 2012, 113(4):737-744.

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